The plates were protected from light and incubated at room temperature for 8 min. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. Using. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 1) EarlyTox Glutathione Assay (6. We would like to show you a description here but the site won’t allow us. SpectraMax® iD5. Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. SoftMax Pro. Impact of fix:Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). In the M2, M5 and Plus 384, the light. Antiretroviral activities. Finally, the absorbance was measured with a SpectraMax M3 microplate reader (Molecular Devices Corporation, Sunnyvale, CA, USA) at a wavelength of 450 nm. Next to the pushbutton is a green LED. Mitosox works fine in 96 wells. Supplier: Molecular Devices. elasticus barks done by associated professor Dr. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. e. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Learn how you can utilize microplate readers to drive landmark discoveries. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Learn how to use the SpectraTest ABS2 Validation Plate, a convenient and reliable tool for verifying the performance of your absorbance microplate reader. 0 mm hex key. . the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. o For example: Ex: 485/Em 525/Cutoff 515 4. 305 reference level saturation. Among the various ready-to-read protocols, users will find pre-written SpectraDrop Plate protocols enabling Micro Volume applications measurement. Detection modalities are shown in Table 1-1. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. This process should take about 3 - 5 minutes. VersaMax ELISA. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. . The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds. Add to Cart. SpectraMax Line of Readers Now Includes 14 Unique Configurations to Accommodate Wide Range of Customer Detection Needs MDS Analytical Technologies Launches Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate ReadersAutomated Functions Autofocus, autoLED intensity, auto-exposure Autofocus Method Image-based autofocus User-trained autofocus Laser autofocus (option)2. Table of Contents. SpectraMax Microplate Readers. , contact Molecular. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The drawing shows a standard microplate of 15 mm height covered with a lid. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. com. Neutrophils were stimulated as described in tissue-culture multi-well plates. Figure 6: Transcreener ADP2 FI assay principle. The cuvette must be in place when you read the plate. Application note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. M2. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is a multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The optical densities (OD) were determined using the SpectraMax ® Plus 384 Microplate Reader (SpectraMax M3; Molecular Devices, SanJosé, CA, USA) at 450 nm and 660 nm [31,34]. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Finally, SpectraMax M3 microplate reader was used to detect 412 nm absorption values, which were positively correlated with GSH levels. The following document is intended to demonstrate setup of this instrument. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Also refer to this Multimode Reader Comparison Chart. Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. 90-0083 series): Excitation 530 nm,. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderWe would like to show you a description here but the site won’t allow us. 0. 313 reference gain check fail. The resulting absorbance values were plotted against time. All the experiments were done in triplicate. 1 release preventing the protocols from being used for validation. 0 out of 5 $ 5,999. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The relative number of viable cells was quantified by measuring the fluorescence (excitation at 560 nm and emission at 590 nm) using a SpectraMax ® M3 (Molecular Devices, San Jose, CA, USA). The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The corrected absorbance is within 5% of the. Immunofluorescence ASC antibody (1:200, Abcam, ab155970, UK) was used for immunofluorescence in P. Male 8 pin mini Din to Female 9D. 304 signal level saturation. . The absorbance was measured at 230. The ALP activity level was determined, relative to that of the control group, as. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Learn more about its features, specifications, and applications in this user. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. The guide also covers the software features, the specifications, and the accessories of the systems. Mitochondrial activity was expressed by the decrease in OD (450–660 nm), discounting the average of the ODs of the controls . For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] i3x Multi-Mode Detection Platform Features User-configurable applications User-configurable detection modules expand the reader’s detection capabilities to. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Bioz Stars score: 86/100, based on 1 PubMed citations. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. In addition, they allow to increase their performance sequentially from models: M2 ‘ M2e;. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. • Tel. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader Biotek Synergy H1 Hybrid Microplate Reader. Acarbose was used as a. gingivalis -treated BMDMs (MOI = 50), with or without pretreatment of OTTSP167 or Compound 50 for 2 h. Click SoftMax Pro Software User Guide. 01) + 0. ; The top 3 importers of Spectramax are India with 365. Cell Degranulation. Using a SpectraMax M3 spectrophotometer from Molecular Devices, we measured the color change at 450 nm. SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Fluorescence Microplate Reader Comparison Chart. Finally, SpectraMax M3 microplate reader was used to detect 412 nm absorption values, which were positively correlated with GSH levels. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 9000-0756. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Fluorescence (FL). SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Lower limits of detection for ATP determined using the ATPlite . Expanded dynamic range. 10. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Description 1. ® reader to the computer. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Activity I Want To Make Friends… And I Need Your Help. Cables. 315 can't find zero order. All configurations. Summary of Contents for Molecular Devices SpectraMax M2. Data were analyzed using Parallel Line Analysis 2. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Accuris SmartDrop. 7°C (gradient heating design). 2 only) SpectraMax M-series Microplate readers. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. To avoid injury, it is recommended that two. {"product":{"total":4,"offset":0,"limit":4,"data":[{"path":"/products/microplate-readers/multi-mode-readers/spectramax-m2-m2e-readers","identifier":"SpectraMax M2/M2e. S. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email. An assay plate containing a 15-point standard curve was read on the SpectraMax. 3: The cuvette chamber. Wafi’s main research topic aims to set up a pharmacodynamic model comparing antibiotic activities against biofilms and examine in parallel their effects on viability and biofilm mass. SpectraMax® M4. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Photometric accuracy. 7°C (gradient heating design). Fluorescent Polarization: Solid black plates, as above. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 2. The drawing shows a standard microplate of 15 mm height covered with a lid. Page 1 SpectraMax ® GEMINI EM Dual-Scanning Microplate Spectrofluorometer Operator’s Manual 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0088 Rev. The concentration of CTC was expressed as mg catechin equivalent per g of extract. Extraction of EPS. SpectraMax i3 Multi-Mode Platform A flexible, user-upgradeable microplate detection system Key Features The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Molecular Devices LLC spectramax m3 multi mode microplate reader spectrophotometer Spectramax M3 Multi Mode Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. 2. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 4. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. spr SpectraMax iD5 SpectraTest ABS2 v1. The exactly same condition without CCK-8 solution was used to subtract background signal that is absorbed by cell medium or natural product. 4. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. SpectraMaxi3Multi. Abstract. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The sample was subsequently washed five times with 200 mL PBS. Spectral Range. 0200-0729. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 1step assay with SpectraMax microplate readers. Table 1. 2. U2OS cell viability (%) = [A cells with different treatments −A contro ]/[A cells with no treatments − A control ] × 100. Sale. The SoftMax® Touch Software is installed in. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. Note: In this user guide, all references to SpectraMax Multi-Mode. The absorbance was read using a UV–visible spectrophotometer (Spectramax M3, Thermo Scientific, Waltham, MA, USA) at a wavelength of 765 nm. 306 plate air cal. 9000-0756. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. spr SpectraMax iD3 SpectraTest ABS2 v1. Multimode Readers Comparison Chart. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Introduction. Incubate at 37ºC for 1–4 hours. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The result was expressed as mg gallic acid. 2. SpectraMax® M5e. Note: you might need to slide the microplate reader on the foam pad to make room for the cytometer. Data for Spectramax M3 Spectrophotometer gathered from related PubMed articles. Page 12 SpectraMax MiniMax Imaging Cytometer Installation Guide 10. INTRODUCTION: Proper physiological function relies on the vascular system to distribute oxygenating blood to all tissues, return deoxygenated blood to the lungs, and maintain tissue homeostasis, including functions such as hemostasis, lipid transport, and immune surveillance. After cooling to room temperature (22 °C), the mixture was transferred to 96-well plates. . . 9000-0756. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:The bound CV was dissolved in 30% acetic acid and the absorption was measured in a microplate reader at OD 590 using SpectraMax M3 (Molecular Devices, United States). Mitochondrial ROS concentration was determined and compared with H 2 O 2 standards using a 96-well black plate and a spectrofluorometer (SpectraMax ® M3; Molecular Devices, San Jose, CA, United States) at 540-nm emission and 580-nm extinction (Maciel et al. Example: [Drawer]. The absorbance wavelengths used were: 286 nm for 4,4′-diapophytoene; 435 nm for 4,4′-diaponeurosporene; 455 nm for 4,4′-diaponeurosporenic acid; and 462 nm for. Polyplex sizes and zeta potentials were measured with a Malvern Instruments Zetasizer (Nano-2590) at a scattering angle of 173°. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. 4. . 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax ABS Plus SpectraTest ABS2 v1. spr SpectraMax Plus 384. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Choose from a three- (M3), four- (M4), orThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 315 can't find zero order. . Results. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. SpectraMax M2, M2e;. 2. Tumorsphere formation assay and limiting dilution assay. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Description 1. 7°C and the bottom to 36. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. 1. Tested w/warranty. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. DevicesThe SpectraMax M2 And M2e Systems Provide The Multi Detection Microplate Readers With Dualmonochromators, Dual-mode Cuvette Ports, With Top & Bottom Reading. 3. ShakerTime: 0 to 999 seconds. Our absorbance plate readers feature our PathCheck Sensor. The optical density was read using a SpectraMax M3 (Molecular Devices, Sunnyvale, CA, USA). Restart the system. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. The DMAC reagent was replaced by EtOH in the control wells. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9 SpectraMax® L (software version 5. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Figure 2-23: Bottle holder To install the bottle holder: 1. Transfection with siRNA. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeNote: In this user guide, SpectraMax ® refers to several SpectraMax . Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 1. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. Add to Cart. , 2020). The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Hydrophobicity assayThese settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Figure 1. Qualify the fluorescence performance of the SpectraMax . A. 312 gain calibration failed. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 1. HNE (EC 3. As for each measurement, I. Transcreener ® ADP. Luciferase Detection Instrumentation Guide. Multimode Reader Comparison Chart. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The detection of intracellular ROS and mitochondrial membrane potential SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. The resorufin product formed was monitored by fluorescence spectroscopy (excitation energy at 530 nm and emission energy at 590 nm) every 10 min in Spectramax M3 (Molecular Devices). Both microplate and cuvette ports can be qualified. At each time point, cells were incubated with CCK-8 reagent for 1 hour at 37 °C, followed by measuring the optical absorbance at 450 nm with the SpectraMax M3 microplate reader (Molecular Devices. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 1. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Fluorescence was quantified on SpectraMax M3 plate reader, Ex 485/Em 520 using SoftMax Pro software. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataProducts : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. SpectraMax® ABS. Background signal was determined by adding 500 U/ml Catalase from Bovine Liver (Sigma-Aldrich, E3289) in one well to convert hydrogen peroxide into. Fluorescence of the samples were read using a SpectraMax M3 instrument with an excitation set to 355 nm and emission set to 460 nm. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 50 0112-0115G Item Description Wavelengthrange 200-1000nm Wavelengthselection Monochromator,tunablein1nmincrementsPage 25 SpectraMax iD3 Multi-Mode Microplate Reader Installation Guide To install the transport lock on the plate drawer: 1. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. We would like to show you a description here but the site won’t allow us. Molecular Devices LLC pro 6 3 software controlled spectramax m3 multimode microplate reader Pro 6 3 Software Controlled Spectramax M3 Multimode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. 72 MB. Other technologies such as imaging. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. In addition, the total flavonoid content was determined using the aluminum chloride method. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. Detection modalities are shown in Table 1-1. SpectraMax® M5. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Molecular Devices Spectramax M3 Mulitmode Microplate Reader is available from The Lab World Group. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. Cells were transfected with Sirt1 siRNA or scrambled siRNA. The spectrophotometer (SpectraMax, M3, Molecular Devices, Washington DC, USA) was used to confirm the encapsulation of gallic acid inside the PLGA nanoparticles. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Please contact Tatiana to reserve the plate reader time: tnl1@psu. D 1 1. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. A single colony of bacterial isolates was inoculated in Marine Broth using a sterile inoculation loop. Italic and boldface type are used for emphasis. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. . SpectraMax M3, M4, and M5/M5e readers. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Add either alarmaBlue HS or alarmaBlue reagent to microplate wells (see recommended volumes) 3. 8. S. Dual monochromators increase performance and assay flexibility. Cables. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 4. SpectraMax M5/M5e/M4/M3/M2/M2e. AquaMax® 4000. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for If application. Total phenolic contents were calculated from the gallic acid standard curve. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. 0 OD for earlier models. Type: Clear: Add to Cart. Both readers feature an 8-channel read head for fast reads and. Operated fromTo ensure loading of equal amounts of protein for each sample, colorimetric protein quantification was performed using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and a SpectraMax M3 spectrophotometer (Molecular Devices, Sunnyvale, CA). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 2. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax DuoLuc Reporter Assay (SMP 6. Wavelength Selection. The luminescent signal was proportional to the amount of GSH or GSSG present. RP-3500 is highly selective for ATR with 30-fold selectivity over mammalian target of rapamycin (mTOR) and more than 2,000-fold selectivity over ataxia. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 190-1000 nm, tunable in 1 nm increments. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 5. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Guanidine hydrochloride at a final concentration of 5 M was used to denature a 10 mg/mL solution of lysozyme. Description 1. Add to Cart. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. Finally, the absorbance was measured with a SpectraMax M3 microplate reader (Molecular Devices Corporation, Sunnyvale, CA, USA) at a wavelength of 450 nm. 313 reference gain check fail. , contact Molecular. Examples: “Press carefully to engage,” “ Do not press down. Also refer to this Multimode Reader Comparison Chart. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The bacterial colony was mixed with. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The information below applies to a standard 96 well plate, set to a linear shake mode. The chamber contains springs that position the cuvette in the proper alignment for a read. $ 15,999. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader.